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KMID : 0614019990150010345
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Journal of Pharmaceutical Sciences (C.N.U.)
1999 Volume.15 No. 1 p.345 ~ p.351
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Transport of Transferrin-Horseradish Peroxidase Conjugate Through Cultured Caco-2 Cell Monolayer
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Kim Dong-Chool
Kim Jie-Hae
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Abstract
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Transport study of horseradish peroxidase and transferrin-horseradish peroxidase conjugate was performed using an in vivo Caco-2 cell cultured monolayer grown on a polycarbonate membrane of Transwell^ⓡ. Horseradish peroxidase was not transported across Caco-2 cell monolayer. Transferrin-horseradish peroxidase conjugate was transported through Caco-2 monolayer. The apparent membrane permeability coefficient(P_app) of transferrin horseradish peroxidase conjugate was 6.54×10^-7cm/sec in the presence of 50μg/ml brefeldin-A. These results suggest the transferrin receptor mediated transcytosis of transferrin-horseradish peroxidase conjugate across Caco-2 cell monolayer.
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KEYWORD
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Caco-2 cell, Transport, Transferrin, Receptor mediated transcytosis
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